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    恒远产品文献:试剂-台盼蓝/台盼兰引用文献

    更新时间:2020-12-24 点击量:949

    【文献标题】Study on antitumor molecular mechanism of Alisols based on p53DNA

    【作者】Fei Xu,Cai Lu, Qinan Wu,et.al

    【作者单位】南京中医药大学(Nanjing University of Chinese Medicine)

    【文献中引用产品】

    试剂-台盼蓝/台盼兰

    【关键词】Alisols,Antitumor activity,Molecular mechanism

    【DOI】doi.org/10.1016/j.ijbiomac.2018.05.158

    【影响因子(IF)】6.90

    出版期刊】《International Journal of Biological Macromolecules》

    【产品原文引用】

    Reagents: DMEM medium and phosphate buffer saline (PBS)

    (HyClone, USA); penicillin and fetal bovine serum (FBS) (Gibco, USA);Methyl thiazolyl tetrazolium (MTT) (Beyotime, China); Dimethyl sulfoxide (DMSO) and trypsin (Amresco, USA); Trypan-blue (Hengyuan Biological Technology, China); Paclitaxel standard (Sigma-Aldrich, USA);human breast cancer cell line (MCF-7) (ASTRI Cell Resource Center,China); Single-stranded DNA (Sangon Biotech, China):P1 promoter region of p53 gene (ss1: 5′-CCTCCTCCCCAACTCC-3′, ss2: 3′-GGAGGAGGGGT TGAGG-5′); Alisol A and alisol B (Mansite Biotechnology,China) (structures shown in Fig. 1 [3]); Ethidium bromide (EB)(Macklin, China); Acetonitrile (LC, Merck, USA); Trihydroxymethyl aminomethane (Tris, BIO LINK, USA); Tris and hydrochloric acid (HCl)were used to prepare Tris-HCl buffer (0.50 mol·L−1), pH 7.4; All other chemicals used were of analytical grade and experimental water for ultrapure water.

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