【文献标题】Effects of pamidronate disodium on the loss of osteoarthritic subchondral bone and the expression of cartilaginous and subchondral osteoprotegerin and RANKL in rabbits
【作者】You Lv，Jie-yun Xia，Jing-yang Chen，et.al
【作者单位】徐州医学大学附属医院（Affiliated Hospital of Xuzhou Medical University）
【文献中引用产品】
试剂-藏红o试剂
试剂-固绿FCF试剂
【关键词】Osteoarthritis, Pamidronate disodium, Subchondral bone, Cartilage, Osteoprotegerin (OPG), Receptor activator of nuclear factor-κ B ligand (RANKL)
【DOI】doi:10.1186/1471-2474-15-370
【影响因子(IF)】1.68
【出版期刊】《BMC Musculoskeletal Disorders》
【产品原文引用】
Rabbits were euthanized [25] and the medial condyles of the femurs were fixed with 4% paraformaldehyde (Boster, Wuhan, China) overnight at 4°C on a shaker.Whole medial condyles were decalcified in 14% ethylenediaminetetraacetic acid for 5 days at 4°C on a shaker. After dehydration by gradient alcohol and infiltration by xylene and paraffin, cartilage samples were embedded in paraffin.Paraffin-embedded joints (4 μm) were sectioned on a sagittal plane. Samples were stained with safranin O (Hengyuan,Shanghai, China) and fast green (Hengyuan) using standard protocols. All sections were evaluated for histologic signs of cartilage degeneration using a modified Osteoarthritis Research Society International (OARSI) scoring system [26].
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